Diagnosis of coeliac disease

Follow up and review are needed when test results are not clear

Two articles in this issue remind us about coeliac disease and the need to consider it as a diagnosis in seemingly unusual circumstances—in elderly patients and in obese patients (p 773, 775).1 2 Furthermore, in the patient reported by Saunders et al, the usual diagnostic features of anti-endomysial and anti-transglutaminase antibodies and histological enteropathy were absent initially.1

Coeliac disease is a relatively common condition. Its prevalence is approximately 0.3-1% of the population in almost all countries and ethnic groups where it has been investigated.3 Previously regarded largely as a childhood problem it is now recognised to affect mostly adults, with about 25% of patients receiving their diagnosis at over 60 years of age.4 When present, the features of malabsorption (diarrhoea and weight loss) should point to the diagnosis, but now a wide range of clinical manifestations are recognised. Patients often have few or no gastrointestinal symptoms and can even be obese.2 5

Conventionally diagnosis is based on the histological finding of villous atrophy in the small bowel, which recovers on a gluten-free diet. Since antibody testing has become available, anti-endomysial and anti-transglutaminase antibodies are often used as a preliminary non-invasive means to screen patients and populations.

Where histological examination of the small bowel shows villous atrophy and antibodiesare found the diagnosis is straightforward. Difficulties arise when one or other of theseis not found, and the diagnosis is particularly difficult when both are negative.1 The objective of diagnosis is to determine treatment, which for coeliac disease is a gluten-free diet. Some patients with coeliac disease have no symptoms and find the limitations imposed by a gluten-free diet difficult to accept. When the diagnosis is in doubt and patients have few or no symptoms, deciding about treatment is more problematic.

Where antibody tests are positive but histological findings are normal, the sensitivity and specificity of antibody testing and the reliability of histological inter-pretation are called into question. Is the antibody test a false positive or the histological examination a false negative? The results of antibody testing in any locale depend on the population used to standardise the test, the particular test used, and the laboratory expertise. In our experience a combination of anti-endomysial and anti-transglutaminase antibodies is highly predictive of the condition (> 95%). We have also seen transiently positive antibody tests, particularly anti-gliadin6 and anti-transglutaminase antibodies, that become negative on retesting and were clearly false positives. By contrast, reports exist of positive antibody testing that predict the development of enteropathy after several years of follow up—for anti-reticulin and anti-gliadin antibodies7 and anti-endomysial antibodies (C Feighery, personal communication, 2005).

Histological assessment of small intestinal biopsies needs expertise. Enteropathy at the mild end of the spectrum—with infiltration by inflammatory cells without villous atrophy (Marsh 1 and 2 lesions)—needs to be recognised.8 Another pitfall is the way in which small intestinal biopsies are prepared. Specimens need to be correctly oriented so that villi are cut longitudinally. If the mucosa is cut tangentially atrophic villi may look normal and normal villi atrophic. The disease may be patchy, so biopsies from several sites (usually four) in the duodenum are recommended. Although coeliac disease is regarded as a proximal small intestinal disorder, it is possible for it to be more manifest distally, and may require enteroscopy for diagnosis.9 Wireless capsule endoscopy may also prove useful for this purpose. Sometimes patients come to the outpatient clinic having started a gluten-free diet after a positive antibody test. If the clinical response has been equivocal and histological findings normal a gluten challenge may help make a diagnosis. This, however, needs further gastroscopy, and uncertainty prevails about when to repeat the biopsy.

What then should be the recommendation for patients with normal histology and a positive test for anti-endomysial or anti-transglutaminase antibodies? The first action should be to review the histology. If the histology shows Marsh 1 or 2 changes and the patient has symptoms treatment could be started. If the histology is normal and the patient has symptoms the options are to treat, to investigate further (for example, with capsule endoscopy or enteroscopy), to review long term, or if the patient has been on a gluten-free diet to review after gluten challenge. If the histology is normal and the patient has no symptoms the options are to review long term or investigate further.

What about patients whose histology is abnormal but who do not have the diagnostic antibodies? The histological appearance of coeliac disease is non-specific and can result from other conditions such as gastroenteritis. Such changes may persist for some time. However, antibody negative cases of coeliac disease do occur,10 and if the condition is suspected clinically, biopsy should still be carried out. A possible reason for negative antibody testing is IgA deficiency. This is more common in coeliac disease and since anti-endomysial and anti-transglutaminase antibodies are normally measured as immunoglobulin A antibodies they will be absent in patients with IgA deficiency. Therefore in patients with IgA deficiency, IgG anti-endomysial and anti-transglutaminase antibodies need to be checked.

Successful diagnosis of coeliac disease depends on a high index of suspicion, careful evaluation of investigations, and, where these are not clear, a willingness to review patients and make the diagnosis later in light of clinical progress and results of re-investigation.

Clinical review pp 773, 775